Exploring the usefulness of loop-mediated isothermal amplification assays: a proof of concept for regulatory plant diagnostics

Funding period: 2022–2023
Lead: Stephan Brière
Total GRDI funding: $9,500

The Ottawa Plant Laboratory's plant pathology lab at the Canadian Food Inspection Agency is currently looking at the potential use of loop-mediated isothermal amplification (LAMP) as a complimentary test method for detecting and identifying plant pathogens. LAMP assays are rapid and low cost and can serve as powerful diagnostic tools when they have the required specificity for regulatory plant health diagnostics.

The main goal of this project is to verify the usefulness, sensitivity and limit of detection of a recently published LAMP assay for the detection of Pseudomonas syringae pv. pisi and pv. syringae. Two additional LAMP assays would be selected for plant pathogenic testing of both bacteria and fungi.

This project explores this proof of concept and, if successful, would lead to a comprehensive research project to evaluate more LAMP assays for these pathogenic bacteria as well as plant fungi and seedborne viruses.

Contact us

For additional information, please contact:
Genomics R&D Initiative
Email: info@grdi-irdg.collaboration.gc.ca