Funding period: 2020-2023
Lead: Oliver Lung
Total GRDI funding: $123,000
The CFIA National Centre for Foreign Animal Disease's (NCFAD) mandate includes surveillance, diagnostic testing, diagnostic test development, and scientific advice regarding transboundary, emerging, and high-consequence animal diseases. NCFAD's Genomics Unit operates a unique high-throughput sequencing (HTS) containment level 3 laboratory (CL3). Currently, HTS is the only available method that allows unequivocal identification and comprehensive genomic characterization of known, novel, and unexpected pathogens as it requires no knowledge of sample identity before testing. Different methods are needed to efficiently obtain viral genome sequences due to the diverse structure of DNA or RNA viral genomes. This project aims to continue to improve quality assurance at Canada's only CL3 HTS laboratory to support diagnostic and surveillance activities and microbial forensic investigations.
Publications
- Berhane, Y, Joseph, T, Lung, O, Embury-Hyatt, C, Xu, W, Cottrell, P, Raverty, S. 2022. Isolation and Characterization of Novel Reassortant Influenza A(H10N7) Virus in a Harbor Seal, British Columbia, Canada. Emerging Infectious Diseases, [online] 28(7), 1480-1484. https://doi.org/10.3201/eid2807.212302
- Caliendo, V, Lewis, NS, Pohlmann, A, Baillie, SR, Banyard, AC, Beer, M, Brown, IH, Fouchier, RAM, Hansen, RDE, Lameris, TK, Lang, AS, Laurendeau, S, Lung, O, Robertson, G, van der Jeugd, H, Alkie, TN, Thorup, K, van Toor, ML, Waldenström, J, Yason, C, Kuiken, T, Berhane, Y. 2022. Transatlantic spread of highly pathogenic avian influenza H5N1 by wild birds from Europe to North America in 2021. Scientific Reports, [online] 12(1). https://doi.org/10.1038/s41598-022-13447-z
- Lung O, Candlish R, Nebroski M, Kruckiewicz P, Buchanan C, Moniwa M. 2021. High-throughput sequencing for species authentication and contamination detection of 63 cell lines. Sci. Rep. 11(1). http://doi.org/10.1038/s41598-021-00779-5
- Pickering, B, Lung, O, Maguire, F, Kruczkiewicz, P, Kotwa, JD, Buchanan, T, Gagnier, M, Guthrie, JL, Jardine, CM, Marchand-Austin, A, Massé, A, McClinchey, H, Nirmalarajah, K, Aftanas, P, Blais-Savoie, J, Chee, HY, Chien, E, Yim, W, Banete, A, Griffin, BD, Yip, L, Goolia, M, Suderman, M, Pinette, M, Smith, G, Sullivan, D, Rudar, J, Vernygora, O, Adey, E, Nebroski, M, Goyette, G, Finzi, A, Laroche, G, Ariana, A, Vahkal, B, Côté, M, McGeer, AJ, Nituch, L, Mubareka, S, Bowman, J. 2022. Divergent SARS-CoV-2 variant emerges in white-tailed deer with deer-to-human transmission. Nature Microbiology, [online] 7(12), 2011-2024. https://doi.org/10.1038/s41564-022-01268-9
Contact us
For additional information, please contact:
Genomics R&D Initiative
Email: info@grdi-irdg.collaboration.gc.ca