Funding period: 2024-2028
Leads: Jun-Jun Liu, Rhiannon Peery
Total GRDI funding: $262,500
The project objectives are: to identify genic variants associated with Quantitative Disease Resistance (QDR) traits through genome-wide association study (GWAS) by re-sequencing the resistome and related candidate genes using an approach of resistance gene enrichment and sequencing (RenSeq); and to develop marker-assisted selection (MAS) tools for selection of QDR.
The expected socio-economic benefits to Canada in the near-term include: broadened genetic base of resistant genotypes for improved genetic diversity and seed collection potential; potential to begin establishment of a second limber pine seed orchard with newly selected resistant genotypes; diversify the array of disease resistance mechanisms in the breeding program; and reference resistome and related genomic resources will benefit novel gene discovery in limber pine breeding and restoration, as well as comparative genomics and evolutionary biology of disease resistance in the host-pathogen systems for related commercial species like western and eastern white pine.
Publications
- Chi, M., Xiang, Y. and Liu, J.J., 2024. Plant thaumatin-like protein family: Genome-wide diversification, evolution, and functional adaptation. In Defense-Related Proteins in Plants (pp. 99-142). Academic Press. https://doi.org/10.1016/B978-0-443-13236-0.00004-X
- Identification of new candidate genes and deployment of their variants for screening limber pine resistance to Cronartium ribicola in the southern Rocky Mountains using an integrated genomics pipeline. British Columbia Regional Meeting, 2024, Canadian Journal of Plant Pathology, Published online: 06 Jan 2025. https://doi.org/10.1080/07060661.2024.2444170
- Liu, J.J., Sniezko, R.A., Houston, S., Krakowski, J., Alger, G., Benowicz, A., Sissons, R., Zamany, A., Williams, H., Kegley, A. and Rancourt, B., 2024. Genome-wide association study reveals polygenic architecture for limber pine quantitative disease resistance to white pine blister rust. Phytopathology®, 114(7), pp.1626-1636. https://doi.org/10.1094/PHYTO-09-23-0338-R
- Screening limber pine genetic resistance against BC coastal races of Cronartium ribicola. British Columbia Regional Meeting, 2024, Canadian Journal of Plant Pathology, Published online: 06 Jan 2025. https://doi.org/10.1080/07060661.2024.2444170
Contact us
For additional information, please contact:
Genomics R&D Initiative
Email: info@grdi-irdg.collaboration.gc.ca