Genomic-enabled analysis of soil biological networks sustaining agro-ecosystem equilibrium

Funding period: 2019-2024
Lead: Lori Phillips
Total GRDI funding: $429,140

Canadian farmers are under increased pressure to produce more food on the same or less land, in a manner that amplifies and sustains production while at same time reducing the environmental footprint. We know that the interactions and activities of soil biological communities support a range of ecosystem goods and services, from enhanced drought resilience through to pathogen suppression. Despite the obvious importance of soil biotic interactions we still do not know what comprises a healthy and resilient biological community, and whether that community can be specifically managed to augment the provision of ecosystem goods and services. We propose to develop an AAFC soil microbiome platform, building off the success of a current Canadian interdepartmental project (EcoBiomics: Metagenomic-Based Ecosystem Biomonitoring; https://grdi.canada.ca/en), to both add value to and facilitate collaborative exploration of these soil microbiome sequencing efforts. Our core objectives are to consolidate current agricultural soil microbiome sequencing datasets into a centralized platform; identify gaps linked to ecosystem goods and services in consolidated sequencing datasets; facilitate inter-operability of soil microbiome datasets; develop bio-banking protocols for soil and soil-DNA to preserve material for future research needs; work with collections experts (e.g. CABI; www.cabi.org) to ensure international recommendations are met; and establish agricultural Genomic Observatories to serve as pillars from which to monitor long-term changes in soil microbiomes in the context of The Global Omics Observatory Network (GLOMICON).

Publications

  • Chen, W, Radford D, Hambleton S. 2022. Towards Improved Detection and Identification of Rust Fungal Pathogens in Environmental Samples Using a Metabarcoding Approach. Phytopathology 112(3), 535-548. http://dx.doi.org/10.1094/PHYTO-01-21-0020-R
  • Drury CF, Reynolds WD, Yang X, McLaughlin NB, Calder W, Phillips LA. 2021. Diverse rotations impact microbial processes, seasonality and overall nitrous oxide emissions from soils. Soil Sci Soc Am J. 85(5):1448–1464. http://dx.doi.org/10.1002/saj2.20298
  • Midwood AJ, Hannam KD, Forge TA, Neilsen D, Emde D, Jones MD. 2020. Importance of drive-row vegetation for soil carbon storage in woody perennial crops: A regional study. Geoderma 377(1):114591. http://doi.org/10.1016/j.geoderma.2020.114591
  • Pérez-Guzmán L, Acosta-Martínez V, Phillips LA, Mauget SA. 2020. Resilience of the microbial communities of semiarid agricultural soils during natural climatic variability events. Appl Soil Ecol. 149:103487. http://dx.doi.org/10.1016/j.apsoil.2019.103487
  • Pérez-Guzmán L, Phillips LA, Acosta-Martínez V. 2021. Comparing biological methods for soil health assessments: EL‐FAME microbial markers, enzyme activities, and taxonomic and functional gene qPCR. Soil Sci Soc Am J. 85:636–653. https://doi.org/10.1002/saj2.20211
  • Pérez-Guzmán L, Phillips LA, Seuradge BJ, Agomoh I, Drury CF, Acosta-Martínez V. 2021. An evaluation of biological soil health indicators in four long-term continuous agroecosystems in Canada. Agrosyst Geosci Environ. 4:e20164. https://doi.org/10.1002/agg2.20164
  • Sharifi M, Phillips L, Zintel S. 2021. Surface-applied organic amendments modify soil biological activity down the soil profile of an irrigated vineyard. Acta Hortic. 1314:487–498. https://doi.org/10.17660/ActaHortic.2021.1314.60
  • Zhang H, Chen W, Zhao B, Phillips LA, Zhou Y, Lapen DR, Liu J. 2020. Sandy soils amended with bentonite induced changes in soil microbiota and fungistasis in maize fields. Applied Soil Ecology 146:103378. https://doi.org/10.1016/j.apsoil.2019.103378
  • Zhang H, Shi Y, Dong Y, Lapen DR, Liu J, Chen W. 2022. Subsoiling and conversion to conservation tillage enriched nitrogen cycling bacterial communities in sandy soils under long-term maize monoculture. Soil Till Res 215. http://dx.doi.org/10.1016/j.still.2021.105197

Contact us

For additional information, please contact:
Genomics R&D Initiative
Email: info@grdi-irdg.collaboration.gc.ca